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The reaction is completed with peroxidase-conjugated anti-human immunoglobulin G and H-diaminobenzidine as substrate-chromogen.The development of one of the seven specific bands on the nitrocellulose membrane is sufficient to consider the sample positive.
The age range was 17 to 71 years (median = 38 years) and 16 to 96 years (median = 44 years) while gender distributions were 50 males and 50 females and 36 males and 34 females for cases and controls, respectively.
Here we report the results of this comparison and some characterization of the response of the patients in relation to number, type, and localization of parasites within the brain.
One hundred patients with NC and 70 controls with other neurological disorders were prospectively studied in the Neurocysticercosis Clinic of the Medical Research Unit for Neurological Diseases at the Hospital of Specialties of the Medical Center of the Social Security Institute of Mexico.
Several methods based on the detection of antibodies against cysticerci in cerebrospinal fluid or serum have been tested.
Among them, an enzyme-linked immunosorbent assay (ELISA) based on the use of a crude parasite antigen has been used by the laboratory network of cysticercosis in Mexico, which has given support to clinicians for up to 7 years.